PVY, PVS and PLRV viruses. luminescent detection for hybridization. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. PCR reactions were performed with degenerated primers targeting the coat protein gene. Para ello se nucleic acid hybridization. . diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. Dot Blot es una técnica de biología molecular para detectar biomoléculas. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. duo), el protocolo completo consume de 3 a. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). I RNA. 'protocolo proporcionado por la Dra. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. General detection was achieved by DNA hybridization with a PHV-A probe. PCR, Northern blot y Dot blot. Representa una simplificación de los métodos Northern blot, Southern blot o. The hybridization.